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KMID : 0942820090080010047
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Journal of Korean Brain Tumor Society
2009 Volume.8 No. 1 p.47 ~ p.51
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In Situ Film Zymography for Evaluation of Matrix Metalloproteinase Activity in Human Glioma
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Jin Shu-Guang
Jung Shin
Moon Kyung-Sub
Kim In-Young
Pei Jian
Jung Tae-Young
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Abstract
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IntroductionGlioma is a common brain tumor, during the initial stage of oncogenesis, there are both invasions of normal tissues nearby and metastasis to contralateral cerebral hemisphere. Among the tumor invasion factors, matrix metalloproteinases(MMPs) facilitates the invasiveness of tumor cells and is highly expressed in malignant brain tumors. This study is to investigate the level of MMP expression, its location in glioma, using in situ film zymography.
Materials and Methods: Thirteen glioblastoma samples and 5 oligodendroglioma samples were studied. Using in situ film zymography method, we classified the MMP expression into 4 patterns, such as tumor center, margin, both(center & margin), and negative.
Results: In glioblastoma samples, 10 samples(76.9%) were positive. Among them, 7 expressed the pattern of margin. In 5 oligodendroglioma samples, only one anaplastic oligodendroglioma which was transformed from oligodendroglioma, expressed as marginal pattern. There was no MMPs expression in normal brain tissues.
Conclusion: The conclusion of this study is that MMPs highly expressed in glioblastoma, and mostly in the pattern of margin. MMPs is thought to be secreted by tumor cells and it may be practical of using MMP inhibitor during the treatment of glioma patients.
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KEYWORD
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Glioma, In situ film zymography, Invasion, Matrix metalloproteinases
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